Appendix :
 

iSect Tools :        








    


  • 1. Preferences :  Edit your preference to set your own values of the image width, the zoom factor, UTR regions and promoter, to hide or show a set of data, as well as to change display shapes, sizes, colors and track spans instead of default settings. It has less priority than the iView Tool.
    


  • 2. Search :  To open a browser window by querying a gene, a BAC, or data (cDNA, T-DNA) and function. For example: psbA or PFG_4A-00715.R.
    


  • 3. Locate :  To open browser window by positiohing its location (chromosome and position).
    


  • 4. Fetch :  To retrieve all displayed data from a chromosome region. For example: 1234-2345 or W/1234-2345.
    


  • 5. mText :  It is now evolved to accept multiple search for both gene list or cDNA/T-DNA/other list to retrieve related or hited data or genes. For example: 

    	please choose type: Gene for gene list or Data for cDNA/T-DNA list.

	psbA
	nad2

	or

	PFG_4A-00715.R
	TTTGCCGACATGCGGAC
    


  • 6. iSectR : iSect (R) tool to retrieve sequence from a specific chromosome region. For example : 1234-2345 or 1234-2345,3456-4567.

    

  • 7. iSectL : iSect (L) tool to retrieve sequences from genome by list of data names or list of genome mapping data. For example:

    	psbA
	PFG_4A-00715.R
	TTTGCCGACATGCGGAC

	or

	query1 chloroplast 0.0 W/1234-2345
	search2 chr01 0.0 C/1234-2345,3456-4567

    

  • 8. PrimerL : iSect primer (L) tool to design primers according to a list of data names existed or a list of genome mapping data. For example:

    	psbA
	PFG_4A-00715.R
	TTTGCCGACATGCGGAC

	or

	query1 chloroplast 0.0 W/1234-2345
	search2 chr01 0.0 C/1234-2345,3456-4567

    

  • 9. PrimerS : iSect primer (S) tool to design primers from user input sequences.  For example:

    	>YourInput.fa
	CTCGCTGGCCTGTTCAGGTGAATCTTAGTATCGTTTTGTGTCCATGTTGCCTCTACTTCG
	CCTGCTTCTGGTGACTTGAATTCTCTCTTTGTTTTGGGCAGCTTCGGGAACCTTCCACAT
	CCGAAGATGCTGAATTCGCTTCTATTTTGCGGACTCTCAAGGACAGTTTTGATAACGCAT
	TCACTGCTATGATTTCCTTCCAGACGAAGAATTCCGAGCGCATATTCAGCACAGGTATAA



  • 10. GeneAtlas : Gene express atlas query tool by probe name or gene name. For example:

    	Qurey:  AFFX-BioB-3_at NULL  001-200-E04 Os07g04850 
	Filter:  GSE7951:stigma ABA treated
	Custom Data: 257 21 567 24 [Signal Stdev ... ]
	Custom Label: My_data_1 Your_data_1 Thee_data_4 ...