1. PrimerS :
Primer size - Opt: Min: Max:
Primer TM - Opt: Min: Max:
GC Content - Min: Max: Clamp:
Primer Zone - Left: Right: Format:     

Please paste your sequences:


  • 1. PrimerS : iSect primer (S) tool to design primers from user input sequences. For example:
    	>YourInput.fa
    	CTCGCTGGCCTGTTCAGGTGAATCTTAGTATCGTTTTGTGTCCATGTTGCCTCTACTTCG
    	CCTGCTTCTGGTGACTTGAATTCTCTCTTTGTTTTGGGCAGCTTCGGGAACCTTCCACAT
    	CCGAAGATGCTGAATTCGCTTCTATTTTGCGGACTCTCAAGGACAGTTTTGATAACGCAT
    	TCACTGCTATGATTTCCTTCCAGACGAAGAATTCCGAGCGCATATTCAGCACAGGTATAA